Colombia face Jamaica in Melbourne on Tuesday in a battle of the underdogs with a quarter-final against England up for grabs.
France will face tournament debutants Morocco later, also in the last 16, as they look to avoid becoming the latest victim of a World Cup upset.
Jamaica have impressed one and all during the tournament in Australia and New Zealand, so far.
Lorne Donaldson´s side had a dispute with their football federation before the mega event over conditions and poor World Cup planning, including a lack of warm-up games.
They had not won a World Cup game prior to this tournament, facing defeat in all three of their matches on their tournament debut in 2019.
"We are going to come up and give it a good shot," Donaldson said on the eve of the last-16 encounter, which kicks off at 0800 GMT.
"Someone will be knocked out, like a boxing match, someone will go down.
"We´re going to be the one standing when the game is over."
Jamaica, ranked 43rd in the world by FIFA, have not conceded a goal in all three matches in the ongoing event and held Brazil and France to goalless draws.
However, they have only notched up one goal, in a 1-0 victory over Panama, and must ensure better service for their prolific Manchester City forward Khadija Shaw.
Colombia will be slight favourites, having defeated Germany 2-1 in the group stage and ranked 25th in the world.
Like Jamaica, they are also looking to qualify for the quarter-finals for the first time.
Their teenage Real Madrid forward Linda Caicedo will be the player to watch out for, having scored twice at the tournament, including a banger against Germany.
She will be supported by fellow 18-year-old Ana Maria Guzman in her tournament debut, with defender Manuela Vanegas suspended after two yellow cards.
Coach Nelson Abadia has mentored both Caicedo and Guzman since the age of 12 and backs them to rise to the occasion.
"I have 23 players and they are all very talented," said Abadia, who is renowned for not making changes to his starting XI.
"I´m fully confident in Ana, she is very capable."